The shooting happened on the northbound lanes of the 14 Freeway at the Avenue L off-ramp just before 3 p.m., according to the California Highway Patrol.
CHP officers were responding to a reported traffic collision when they found the body of 37-year-old Henry Gonzalez at the scene, where he was pronounced dead.
Sun Valley residents Isabel Cortez, 25, and Jesse Cupido, 29 were arrested Friday on suspicion of murder, CHP Officer April Elliott said. The CHP gave no details on what led up to the arrests.
Anyone with information on the killing was asked to call the CHP at 323-644-9550. Tipsters can also call Crime Stoppers at 800-222-8477.