Las Vegas Metropolitan Police Department officers responded to a shooting in a hotel room on the 8th floor, Las Vegas affiliate KLAS reported.
Police said the victims and the suspect were known to each other.
Videos taken at the scene showed a security officer preventing customers from entering or leaving the hotel. The officer claimed the hotel was on lockdown following a "critical incident."
Las Vegas police said that the arrest was made within six hours of the shooting.
A heavy police presence was seen outside the hotel and police encouraged people to avoid the area.
Police said the shooting at the iconic Las Vegas hotel was "an isolated event."